Download PDF by Cheryl L. Scudamore: A Practical Guide to the Histology of the Mouse

By Cheryl L. Scudamore

ISBN-10: 1119941202

ISBN-13: 9781119941200

A sensible advisor to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse versions of illness are used commonly in biomedical learn with many hundreds and hundreds of latest types being generated every year. entire phenotypic research of all of those types can reap the benefits of histologic overview of the tissues.

This e-book is aimed toward veterinary and clinical pathologists who're unexpected with mouse tissues and scientists who desire to assessment their very own mouse types. It presents functional tips at the assortment, sampling and research of mouse tissue samples to be able to maximize the knowledge that may be received from those tissues. in addition to illustrating the traditional microscopic anatomy of the mouse, the ebook additionally describes and explains the typical anatomic adaptations, artefacts linked to tissue assortment and history lesions to aid the scientist to tell apart those alterations from experimentally- triggered lesions.

This should be a vital bench-side significant other for researchers and practitioners trying to find an obtainable and well-illustrated advisor to mouse pathology.

  • Written by means of skilled pathologists and particularly adapted to the desires of scientists and histologists
  • Full color throughout
  • Provides recommendation on sampling tissues, necropsy and recording data
  • Includes universal anatomic diversifications, historical past lesions and artefacts so that it will support non-experts comprehend even if histologic diversifications noticeable are a part of the conventional historical past or concerning their experimental manipulation

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Additional info for A Practical Guide to the Histology of the Mouse

Sample text

All tissues should be fixed as soon as possible. Recommended fixatives include 10% neutral buffered formalin or 4% paraformaldehyde (Cardiff et al. 2000) or modified Davidson’s solution for the fixing of testes and eyes. Approximately 20 times the volume of fixative should be used to the volume of the tissues to obtain optimal fixation (Seymour et al. 2004). Retaining the remainder of the carcass in formalin is good practice and will allow further analysis if necessary, for example examination of the nasal cavity if rhinitis is suspected or examination of tympanic bullae for otitis media.

Illustrations © Veterinary Path Illustrations, unless stated otherwise. Published 2014 by John Wiley & Sons, Ltd. 1 Comparison of diagnostic and experimental approaches to reporting pathology data adapted from Holland 2011. Diagnostic approach Experimental approach Expecting to find pathology to support a ‘diagnosis’ No expectation of identifying specific findings. g. from the literature and previous experience) Also uses knowledge from concurrent controls and historical controls from similar strain May produce a definitive, differential diagnosis or a diagnosis based on exclusion Result is a list of difference between controls and treatment group animals Rarely peer reviewed, but may request second opinion Often peer reviewed (toxicological pathology) Qualitative narrative report Semiquantitative data presented in form of incidence and severity tables No statistical analysis required or feasible Statistical analysis is feasible and may be applied/necessary scoring or quantitative methodology to enrich the data.

Jikken Dobutsu 26, 149–159. R. F. (1978a) Scanning electron microscopy of early postmortem artefacts in the small intestine of a neonatal calf. British Journal of Experimental Pathology 59, 499–503. R. F. (1978b) The rate of development of postmortem artefact in the small intestine of neonatal calves. British Journal of Experimental Pathology 59, 178–182. , Poitout-Belissent, F. et al. (2011) Bone Marrow Working Group of ASVCP/STP. Best practices for evaluation of bone marrow in nonclinical toxicity studies.

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A Practical Guide to the Histology of the Mouse by Cheryl L. Scudamore

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