By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the difficult artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide a number of issues in pluripotent stem mobile culturing together with mouse and human fibroblasts, human embryonic stem cells and triggered pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a entire number of illustrated recommendations complemented via informative and academic captions analyzing what high quality cells seem like and the way they behave in numerous environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and brought about pluripotent stem mobile colonies. This distinctive and thorough atlas is a useful source for researchers, academics, and scholars who're drawn to or operating with stem telephone culturing.
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Extra resources for Atlas of Human Pluripotent Stem Cells in Culture
Comparative study of mouse and human feeder cells for human embryonic stem cells. Int J Dev Biol. 2008;52:353–63. 3 Inactivated Mouse and Human Fibroblasts To date, the use of inactivated fibroblasts in the derivation and culture of pluripotent stem cells (PSCs) remains the most widely used culture system despite the availability of other systems that are feeder-free. In general, the feeder-based systems tend to be less expensive than their feeder-free equivalents, and feeders have a proven ability to maintain PSCs a stably in an undifferentiated state, so in most cases the costeffective feeder system is the system of choice.
In general, the feeder-based systems tend to be less expensive than their feeder-free equivalents, and feeders have a proven ability to maintain PSCs a stably in an undifferentiated state, so in most cases the costeffective feeder system is the system of choice. This chapter looks at the morphology of mouse and human feeder cells in order make a subjective assessment of the quality of the feeders and their suitability to support the growth and expansion of PSCs (Figs. 9). b Fig. 1 (a, b) Mouse embryonic fibroblast (MEF) feeder layers of very good quality.
1–17. Nieto A, Cabrera CM, Catalina P, Cobo F, Barnie A, Cortés JL, et al. Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry. Cell Biol Int. 2007;31:269–78. Prathalingam N, Ferguson L, Young L, Lietz G, Oldershaw R, Healy L, et al. Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture.
Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban